Background:
The reactivity of the antiserum is restricted to determinants on the CH2 domain of IgA. It reacts with both subclasses of IgA as tested in haemagglutination, haemagglutination inhibition, indirect binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of IgA in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgA, haemagglutination and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1000 upwards. These data should be interpreted as general recommendations only.
Clone: NI 69 (A89-034) and NI 184 (A89-035)
Isotype: Mouse IgG1
Source: Highly purified monoclonal IgA isolated from pooled human serum
Reactivity: human. The anti-serum does not react with any other component of the human Ig system or any other human plasma protein as tested. This anti-serum has not been tested for cross-reactivity with other species.
Specificity: Monoclonal Mouse anti-serum to Human IgA, isotype specific
Form: Delipidated, heat inactivated, lyophilized, stable whole ascites. IgG concentration is 1 mg/ml. No foreign proteins added.
Applications: Indirect immunofluorescence, ELISA
Storage: Store at 4C, or in small aliquots at -20C.
