IgA2 (subclass specific) (clone NI 512 (A89-038)) HRP, anti-human
€824.00
In stock
SKU
ARP-21-5116
Catalog Number: 21-5116
Size: 200 µg
Isotype: Mouse IgG1
Size: 200 µg
Isotype: Mouse IgG1
Background:
The reactivity of the antiserum is restricted to a subclass specific determinant on the IgA2 molecule as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of IgA2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgA2, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 to 1:100; in ELISA from 1:50 upwards; in Western blotting from 1:100 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.
Clone: NI 512 (A89-038)
Isotype: Mouse IgG1
Source: Highly purified monoclonal IgA2 isolated from pooled human serum.
Reactivity: human. The anti-serum does not react with any other component of the human Ig system or any other human plasma protein as tested. This anti-serum has not been tested for crossreactivity with other species.
Specificity: Horseradish peroxidase conjugated purified monoclonal Mouse antibody to Human IgA2, subclass specific
Form: Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 0.4 mg/ml. Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. No foreign proteins added.
Applications: ELISA, Immunocytochemistry, Immunohistochemistry (paraffin), Dot blot, Immunoblotting
Storage: Store at 4C, or in small aliquots at -20C.
The reactivity of the antiserum is restricted to a subclass specific determinant on the IgA2 molecule as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of IgA2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgA2, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 to 1:100; in ELISA from 1:50 upwards; in Western blotting from 1:100 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.
Clone: NI 512 (A89-038)
Isotype: Mouse IgG1
Source: Highly purified monoclonal IgA2 isolated from pooled human serum.
Reactivity: human. The anti-serum does not react with any other component of the human Ig system or any other human plasma protein as tested. This anti-serum has not been tested for crossreactivity with other species.
Specificity: Horseradish peroxidase conjugated purified monoclonal Mouse antibody to Human IgA2, subclass specific
Form: Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 0.4 mg/ml. Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. No foreign proteins added.
Applications: ELISA, Immunocytochemistry, Immunohistochemistry (paraffin), Dot blot, Immunoblotting
Storage: Store at 4C, or in small aliquots at -20C.
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