IgD (class specific) (clone NI 158), anti-human

Background:
The reactivity of the antiserum is restricted to the IgD molecule as tested in ELISA, immunoblotting, immunoprecipitation. Precipitation takes place under special test conditions in the presence of polyethylene glycol To identify the presence of IgD in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgD immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100; in ELISA from 1:25 upwards; in Western blotting from 1:50 upwards. These data should be interpreted as general recommendations only.

Clone: NI 158

Isotype: Mouse IgG1

Source: Highly purified monoclonal IgD isolated from human serum

Reactivity: human. The anti-serum does not react with any other component of the human Ig system or any other human plasma protein as tested. This anti-serum has not been tested for cross-reactivity with other species.

Specificity: Purified monoclonal Mouse antibody to Human IgD, isotype specific

Form: Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2). IgG concentration is 0.4 mg/ml. No foreign proteins added.

Applications: Indirect immunofluorescence, ELISA

Storage: Store at 4C, or in small aliquots at -20C.