IgE (class specific) (clone NI 307) biotin, anti-human

Background:
The reactivity of the antiserum is restricted to an isotype specific determinant of the IgE molecule as tested in direct binding enzyme immunoassay, immunoblotting, immuno- precipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgE, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only. Working dilutions may vary widely, strongly depending on the test conditions

Clone: NI 307

Isotype: Mouse IgG1

Source: Highly purified polyclonal IgE isolated from human serum.

Reactivity: human. The anti-serum does not react with any other component of the human Ig system or any other human plasma protein as tested. This anti-serum has not been tested for cross- reactivity with other species.

Specificity: Biotin-conjugated purified monoclonal Mouse antibody to Human IgE, isotype specific

Form: Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Applications: ELISA, Immunocytochemistry, Immunohistochemistry (paraffin), Dot blot, Immunoblotting

Storage: Store at 4C, or in small aliquots at -20C.