IgE (class specific) (clone NI 307) HRP, anti-human

Background:
The reactivity of the this monoclonal antibody is restricted to polyclonal and monoclonal IgE, as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic immunoglobulins. To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:250; in ELISA from 1:250 upwards; in Western blotting from 1:500 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.

Clone: NI 307

Isotype: Mouse IgG1

Source: Highly purified monoclonal IgE isolated from human serum.

Reactivity: human. The antibody does not react with any other component of the human immunoglobulin system or any other human plasma protein as tested. This anti-serum has not been tested for cross-reactivity with other species.

Specificity: Horseradish peroxidase conjugated purified monoclonal Mouse antibody to Human IgE, isotype specific.

Form: Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 0.4 mg/ml. Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. No foreign proteins added.

Applications: ELISA, Immunocytochemistry, Immunohistochemistry (paraffin), Dot blot, Immunoblotting

Storage: Store at 4C, or in small aliquots at -20C.