IgG (class specific) (clone NI 335 and NI 343) HRP, anti-human
€824.00
In stock
SKU
ARP-21-5134
Catalog Number: 21-5134
Size: 200 µg
Isotype: Mouse IgG1
Size: 200 µg
Isotype: Mouse IgG1
Background:
The reactivity of the this monoclonal antibody is restricted to isotype specific determinants on the Fc part of IgG. The antiserum reacts with the 4 subclasses of human IgG, as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic immunoglobulins. To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:500; in ELISA from 1:1000 upwards; in Western blotting from 1:2000 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.
Clone: NI 335 and NI 343
Isotype: Mouse IgG1
Source: Highly purified polyclonal IgG isolated from human serum.
Reactivity: human. The antibody does not react with any other component of the human immunoglobulin system or any other human plasma protein as tested. This anti-serum has not been tested for cross-reactivity with other species.
Specificity: Horseradish peroxidase conjugated purified monoclonal Mouse antibody to Human IgG, isotype specific.
Form: Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
Applications: ELISA, Immunocytochemistry, Immunohistochemistry (paraffin), Dot blot, Immunoblotting
Storage: Store at 4C, or in small aliquots at -20C.
The reactivity of the this monoclonal antibody is restricted to isotype specific determinants on the Fc part of IgG. The antiserum reacts with the 4 subclasses of human IgG, as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic immunoglobulins. To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:500; in ELISA from 1:1000 upwards; in Western blotting from 1:2000 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.
Clone: NI 335 and NI 343
Isotype: Mouse IgG1
Source: Highly purified polyclonal IgG isolated from human serum.
Reactivity: human. The antibody does not react with any other component of the human immunoglobulin system or any other human plasma protein as tested. This anti-serum has not been tested for cross-reactivity with other species.
Specificity: Horseradish peroxidase conjugated purified monoclonal Mouse antibody to Human IgG, isotype specific.
Form: Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
Applications: ELISA, Immunocytochemistry, Immunohistochemistry (paraffin), Dot blot, Immunoblotting
Storage: Store at 4C, or in small aliquots at -20C.
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