IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker) (clone IG217 + IG266), anti-human

IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker) (clone IG217 + IG266), anti-human

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In stock
SKU
ARP-24-3500-MSM3
Catalog Number: 24-3500-MSM3
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG1 + IgG2a, kappa
Decription:
This monoclonal antibody is specific to heavy chain of IgG and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with all subclasses of γ heavy chain (IgG1, IgG2a, IgG2b, IgG3). The most common feature of plasmacytomas, and certain non-Hodgkin�s lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Ab-3 is superb for staining of formalin-fixed tissues.

Synonyms: G1m Marker, G2m Marker, G3m Marker, G4m Marker, HDC, Heavy Chain Disease Protein, Human Immunglobulin G, Ig gamma1/2/3/4 Chain C Region, IGHG1, IGHG2, IGHG3, IGHG4, Immunoglobulin Heavy Constant  1/2/3/4

Clone: IG217 + IG266

Isotype: Mouse IgG1 + IgG2a, kappa

Immunogen: Purified human IgG

Reactivity: human. Others not known.

Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Applications: Flow Cytometry (1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Reactivity: 293T, Raji or hPBL cells. Tonsil or Spleen.

Concentration: 0.2mg/ml

Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
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