IgG2 (Fab subclass specific) (clone NI 6014 (HP 6014)), anti-human
€704.00
In stock
SKU
ARP-21-5143
Catalog Number: 21-5143
Size: 200 µg
Isotype: Mouse IgG1
Size: 200 µg
Isotype: Mouse IgG1
Background:
The reactivity of the antiserum is restricted to a subclass specific determinant on the Fab part of the IgG2 molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG2, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
Clone: NI 6014 (HP 6014)
Isotype: Mouse IgG1
Source: Highly purified monoclonal IgG2 isolated from human serum
Reactivity: human. The anti-serum does not react with any other component of the human Ig system or any other human plasma protein as tested. This anti-serum has not been tested for cross-reactivity with other species.
Specificity: Monoclonal Mouse antibody to Human IgG2, subclass specific.
Form: Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 0.4 mg/ml. No foreign proteins added.
Applications: Indirect immunofluorescence, ELISA
Storage: Store at 4C, or in small aliquots at -20C.
The reactivity of the antiserum is restricted to a subclass specific determinant on the Fab part of the IgG2 molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG2, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
Clone: NI 6014 (HP 6014)
Isotype: Mouse IgG1
Source: Highly purified monoclonal IgG2 isolated from human serum
Reactivity: human. The anti-serum does not react with any other component of the human Ig system or any other human plasma protein as tested. This anti-serum has not been tested for cross-reactivity with other species.
Specificity: Monoclonal Mouse antibody to Human IgG2, subclass specific.
Form: Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 0.4 mg/ml. No foreign proteins added.
Applications: Indirect immunofluorescence, ELISA
Storage: Store at 4C, or in small aliquots at -20C.
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