IgG2 (Fab subclass specific) (clone NI 6014 (HP 6014)) HRP, anti-human
€824.00
In stock
SKU
ARP-21-5146
Catalog Number: 21-5146
Size: 200 µg
Isotype: Mouse IgG1
Size: 200 µg
Isotype: Mouse IgG1
Background:
The reactivity of the antiserum is restricted to a subclass specific determinant on the Fab portion of the IgG2 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:25 to 1:100; in ELISA from 1:250 upwards; in Western blotting from 1:500 upwards. These data should be interpreted as general recommendations only.
Clone: NI 6014 (HP 6014)
Isotype: Mouse IgG1
Source: Highly purified monoclonal IgG2 isolated from pooled human serum
Reactivity: human. The anti-serum does not react with any other component of the human Ig system or any other human plasma protein as tested. This anti-serum has not been tested for crossreactivity with other species.
Specificity: Peroxidase conjugated purified monoclonal Mouse antibody to Human IgG2, subclass specific.
Form: Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
Applications: ELISA, Immunocytochemistry, Immunohistochemistry (paraffin), Dot blot, Immunoblotting
Storage: Store at 4C, or in small aliquots at -20C.
The reactivity of the antiserum is restricted to a subclass specific determinant on the Fab portion of the IgG2 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:25 to 1:100; in ELISA from 1:250 upwards; in Western blotting from 1:500 upwards. These data should be interpreted as general recommendations only.
Clone: NI 6014 (HP 6014)
Isotype: Mouse IgG1
Source: Highly purified monoclonal IgG2 isolated from pooled human serum
Reactivity: human. The anti-serum does not react with any other component of the human Ig system or any other human plasma protein as tested. This anti-serum has not been tested for crossreactivity with other species.
Specificity: Peroxidase conjugated purified monoclonal Mouse antibody to Human IgG2, subclass specific.
Form: Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
Applications: ELISA, Immunocytochemistry, Immunohistochemistry (paraffin), Dot blot, Immunoblotting
Storage: Store at 4C, or in small aliquots at -20C.
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