IgG2 (Fc subclass specific) (clone NI 25-1 (HP 6207)) FITC, anti-human
€824.00
In stock
SKU
ARP-21-5151
Catalog Number: 21-5151
Size: 200 µg
Isotype: Mouse IgG1
Size: 200 µg
Isotype: Mouse IgG1
Background:
The reactivity of the antiserum is restricted to a subclass specific determinant on the Fc part of the IgG2 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:50; in ELISA from 1:200 upwards; in Western blotting from 1:400 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.
Clone: NI 25-1 (HP 6207)
Isotype: Mouse IgG1
Source: Highly purified monoclonal IgG2 isolated from human serum.
Reactivity: human. The anti-serum does not react with any other component of the human Ig system or any other human plasma protein as tested. This anti-serum has not been tested for cross-reactivity with other species.
Specificity: Fluorescein isothiocyanate-conjugated purified monoclonal Mouse antibody to Human IgG2, subclass specific.
Form: Purified monoclonal mouse IgG1 kappa conjugated with FITC, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 0.4 mg/ml. Fluorochrome/IgG protein molar ratio (F/P) approximately 1.5. No foreign proteins added.
Applications: ELISA, Immunocytochemistry, Immunohistochemistry (frozen), (In)direct immunofluorescence
Storage: Store at 4C, or in small aliquots at -20C.
The reactivity of the antiserum is restricted to a subclass specific determinant on the Fc part of the IgG2 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:50; in ELISA from 1:200 upwards; in Western blotting from 1:400 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.
Clone: NI 25-1 (HP 6207)
Isotype: Mouse IgG1
Source: Highly purified monoclonal IgG2 isolated from human serum.
Reactivity: human. The anti-serum does not react with any other component of the human Ig system or any other human plasma protein as tested. This anti-serum has not been tested for cross-reactivity with other species.
Specificity: Fluorescein isothiocyanate-conjugated purified monoclonal Mouse antibody to Human IgG2, subclass specific.
Form: Purified monoclonal mouse IgG1 kappa conjugated with FITC, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 0.4 mg/ml. Fluorochrome/IgG protein molar ratio (F/P) approximately 1.5. No foreign proteins added.
Applications: ELISA, Immunocytochemistry, Immunohistochemistry (frozen), (In)direct immunofluorescence
Storage: Store at 4C, or in small aliquots at -20C.
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