IgG3 (F(ab'')2 subclass specific) (clone NI 330 (HP 6050)), anti-human
€624.00
In stock
SKU
ARP-21-5154
Catalog Number: 21-5154
Size: 500 µg
Isotype: Mouse IgG1
Size: 500 µg
Isotype: Mouse IgG1
Background:
The reactivity of the antiserum is restricted to a subclass specific determinant on the F(ab')2 portion of the IgG3 molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG3, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
Clone: NI 330 (HP 6050)
Isotype: Mouse IgG1
Source: Highly purified monoclonal IgG1 kappa isolated from pooled human serum
Reactivity: human. The anti-serum does not react with any other component of the human Ig system or any other human plasma protein as tested. This anti-serum has not been tested for cross-reactivity with other species.
Specificity: Monoclonal Mouse antibody to Human IgG3, subclass specific.
Form: Delipidated, heat inactivated lyophilized stable ascites. No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration after solution is 1.0 mg/ml. No foreign proteins added.
Applications: Indirect immunofluorescence, ELISA
Storage: Store at 4C, or in small aliquots at -20C.
The reactivity of the antiserum is restricted to a subclass specific determinant on the F(ab')2 portion of the IgG3 molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG3, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
Clone: NI 330 (HP 6050)
Isotype: Mouse IgG1
Source: Highly purified monoclonal IgG1 kappa isolated from pooled human serum
Reactivity: human. The anti-serum does not react with any other component of the human Ig system or any other human plasma protein as tested. This anti-serum has not been tested for cross-reactivity with other species.
Specificity: Monoclonal Mouse antibody to Human IgG3, subclass specific.
Form: Delipidated, heat inactivated lyophilized stable ascites. No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration after solution is 1.0 mg/ml. No foreign proteins added.
Applications: Indirect immunofluorescence, ELISA
Storage: Store at 4C, or in small aliquots at -20C.
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