IgG4 (pFc subclass specific) (clone NI 315 (HP 6206)), anti-human

Background:
The reactivity of the antiserum is restricted to a subclass specific determinant on the pFc part of the IgG4 molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG4, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Clone: NI 315 (HP 6206)

Isotype: Mouse IgG1

Source: Highly purified monoclonal IgG4 isolated from human serum

Reactivity: human. The anti-serum does not react with any other component of the human Ig system or any other human plasma protein as tested. This anti-serum has not been tested for cross-reactivity with other species

Specificity: Monoclonal Mouse antibody to Human IgG4, subclass specific.

Form: Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 2.0 mg/ml. No foreign proteins added.

Applications: Indirect immunofluorescence, ELISA

Storage: Store at 4C, or in small aliquots at -20C.