IgM (class specific) (cloneNI 179 ), anti-human

Background:
The reactivity of the antiserum is restricted to an isotype specific determinant of the IgM molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgM, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Clone: NI 179

Isotype: Mouse IgG1

Source: Highly purified monoclonal IgM isolated from pooled human serum

Reactivity: human. The anti-serum does not react with any other component of the human Ig system or any other human plasma protein as tested. This anti-serum has not been tested for cross-reactivity with other species.

Specificity: Monoclonal Mouse antibody to Human IgM, isotype specific.

Form: Delipidated, heat inactivated lyophilized stable ascites. No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 1.0 mg/ml. No foreign proteins added.

Applications: Indirect immunofluorescence, ELISA

Storage: Store at 4C, or in small aliquots at -20C.