IHH human active recombinant protein

IHH human active recombinant protein

€295.00
In stock
SKU
600393
Catalog Number: 600393
Size: 0.025 mg
Datasheet
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Protein Family: Peptidases

Pathway and Disease: Cell Growth and Death, Endocrine System, Signal Transduction, Signaling Molecules and Interaction

Alternate Names: HHG-2, Indian Hedgehog protein, IHHDescription:
Indian Hedgehog (IHH) is a developmental factor found in the gut, cartilage and bones of an embryo. IHH acts through the patched (PTC) receptor and induces transcriptional changes important for bone development and include the up regulation of parathyroid hormone-related protein (PTHRP). Human IHH is produced in E. coli as a non-glycosylated protein, containing 177 amino acids, with a molecular weight of 20 kDa.

Source: E. coli. Endotoxin level as measured by LAL is <0.05ng/ug or <0.5EU/ug.

Applications: E, WB

Application Notes:
The activity is determined by the dose-dependent induction of alkaline phosphatase activity of CCL-226 cells and is typically less than 10 ug/mL. Specific activity: 1 x 10e2 units/mg

Accession No.: Q14623

MW: 20000 Da

Sequence:
MIIGPGRVVG SRRRPPRKLV PLAYKQFSPN VPEKTLGASG RYEGKIARSS ERFKELTPNY NPDIIFKDEE NTGADRLMTQ RCKDRLNSLA ISVMNQWPGV KLRVTEGWDE DGHHSEESLH YEGRAVDITT SDRDRNKYGL LARLAVEAGF DWVYYESKAH VHCSVKSEHS AAAKTGG

Purity:
Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Format:
Each vial contains 25 ug of lyophilized protein. Reconstitute with 0.25 ml sterile deionized water for a final concentration of 0.1 mg/ml.

Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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