IL-1 beta human active recombinant protein
€295.00
In stock
SKU
600318
Protein Family: Cytokines
Pathway and Disease: Cell Growth and Death, Immune System, Metabolic Disorders, Neurodegenerative Disorders, Signal Transduction, Signaling Molecules and Interaction
Alternate Names: Catabolin, LAF, EP, LEM, MCFDescription:
Interleukin-1 beta (IL-1beta) is a pro-inflammatory cytokine produced in response to inflammatory agents by a variety of cells, including monocytes, macrophages, and dendritic cells (DCs). IL-1beta and IL-1alpha are two distinct and independently regulated gene products that comprise IL-1 and signal through the Type 1 IL-1 receptor (IL-1R1). Although IL-1alpha is cell associated and IL-1beta is secreted, they have nearly identical biological activity in that they induce adhesion molecule expression on epithelial cells, control fever induction, and play a role in arthritis and septic shock. Signaling activated by the IL-1R1 promotes these activities through a MYD88 signaling pathway similar to those associated with Toll receptors. Human IL-1 beta is produced in E.coli. Recombinant human IL-1beta is a non-glycosylated protein, containing 153 amino acids, with a molecular weight of 17.4 kDa.
Source: E. coli. Endotoxin level as measured by LAL is <0.05ng/ug or <0.5EU/ug.
Applications: E, WB
Application Notes:
The activity is determined by the stimulation of D10S cells and is typically less than 1 pg/mL. Specific activity: 8 x 10e7 units/mg
Accession No.: P01584
MW: 17377 Da
Sequence:
APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS
Purity:
Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 10 ug of lyophilized protein. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathway and Disease: Cell Growth and Death, Immune System, Metabolic Disorders, Neurodegenerative Disorders, Signal Transduction, Signaling Molecules and Interaction
Alternate Names: Catabolin, LAF, EP, LEM, MCFDescription:
Interleukin-1 beta (IL-1beta) is a pro-inflammatory cytokine produced in response to inflammatory agents by a variety of cells, including monocytes, macrophages, and dendritic cells (DCs). IL-1beta and IL-1alpha are two distinct and independently regulated gene products that comprise IL-1 and signal through the Type 1 IL-1 receptor (IL-1R1). Although IL-1alpha is cell associated and IL-1beta is secreted, they have nearly identical biological activity in that they induce adhesion molecule expression on epithelial cells, control fever induction, and play a role in arthritis and septic shock. Signaling activated by the IL-1R1 promotes these activities through a MYD88 signaling pathway similar to those associated with Toll receptors. Human IL-1 beta is produced in E.coli. Recombinant human IL-1beta is a non-glycosylated protein, containing 153 amino acids, with a molecular weight of 17.4 kDa.
Source: E. coli. Endotoxin level as measured by LAL is <0.05ng/ug or <0.5EU/ug.
Applications: E, WB
Application Notes:
The activity is determined by the stimulation of D10S cells and is typically less than 1 pg/mL. Specific activity: 8 x 10e7 units/mg
Accession No.: P01584
MW: 17377 Da
Sequence:
APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS
Purity:
Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 10 ug of lyophilized protein. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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