IL-3 mouse active recombinant protein

IL-3 mouse active recombinant protein

€295.00
In stock
SKU
600190
Catalog Number: 600190
Size: 0.01 mg
Datasheet
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Protein Family: Cytokines

Pathway and Disease: Immune System, Signal Transduction, Signaling Molecules and Interaction

Alternate Names: Interleukin-3, IL-3, multipotential colony-stimulating factor, hematopoietic growth factor, P-cell-stimulating factor, mast cell growth factor, MCGF, IL3, CsfmuDescription:
Interleukin-3 (IL-3) is a cytokine produced by activated T-cells and mast cells that is active on a broad range of hematopoietic cells and in the nervous system. IL-3 is important in several chronic inflammatory diseases. Recombinant mouse IL-3 produced in E. coli is a single non-glycosylated polypeptide chain containing 135 amino acids with a MW of 15,100 Da.

Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Applications: E, WB

Application Notes:
Mouse IL-3 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of mouse M-NFS-60 cells is 0.04 ng/ml.

Accession No.: P01586

MW: 15100 Da

Sequence:
MDTHRLTRTL NCSSIVKEII GKLPEPELKT DDEGPSLRNK SFRRVNLSKF VESQGEVDPE DRYVIKSNLQ KLNCCLPTSA NDSALPGVFI RDLDDFRKKL RFYMVHLNDL ETVLTSRPPQ PASGSVSPNR GTVEC

Purity:
Purity > 97% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Format:
Each vial contains 10 ug of lyophilized protein. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.

Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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