IL-32 alpha human active recombinant protein
€295.00
In stock
SKU
600144
Protein Family: Cytokines
Pathway and Disease: Immune System, Signal Transduction, Signaling Molecules and Interaction
Alternate Names: Interleukin-32, IL-32, natural killer cells protein 4, tumor necrosis factor alpha-inducing factorDescription:
Interleukin-32 alpha (IL-32 alpha) is one of approximately 6 splice variants of a gene cloned from the human lung carcinoma stable transfectant, A549-Rbeta. IL-32 alpha has been shown to induce IL-8, TNF-alpha, and MIP-2 production from human and mouse macrophage cell lines. IL-32 alpha is upregulated in activated T- & NK-cells, and IFN-gamma-treated epithelial cells. Human recombinant IL-32 alpha produced in E. coli is a 131 amino acid non-glycosylated polypeptide with a MW of 14.9 kDa.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
Human IL-32 alpha activity is measured via the dose-dependent induction of TNF-alpha production from human PBMCs. The ED50 for this assay is in the range of 0.125-1.0ug/ml.
Accession No.: P24001
MW: 14900 Da
Sequence:
MCFPKVLSDD MKKLKARMHQ AIERFYDKMQ NAESGRGQVM SSLAELEDDF KEGYLETVAA YYEEQHPELT PLLEKERDGL RCRGNRSPVP DVEDPATEEP GESFCDKSYG APRGDKEELT PQKCSEPQSS K
Purity:
Purity > 97% as determined by reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 10 ug protein lyophilized in 50 mM sodium phosphate pH7.5. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathway and Disease: Immune System, Signal Transduction, Signaling Molecules and Interaction
Alternate Names: Interleukin-32, IL-32, natural killer cells protein 4, tumor necrosis factor alpha-inducing factorDescription:
Interleukin-32 alpha (IL-32 alpha) is one of approximately 6 splice variants of a gene cloned from the human lung carcinoma stable transfectant, A549-Rbeta. IL-32 alpha has been shown to induce IL-8, TNF-alpha, and MIP-2 production from human and mouse macrophage cell lines. IL-32 alpha is upregulated in activated T- & NK-cells, and IFN-gamma-treated epithelial cells. Human recombinant IL-32 alpha produced in E. coli is a 131 amino acid non-glycosylated polypeptide with a MW of 14.9 kDa.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
Human IL-32 alpha activity is measured via the dose-dependent induction of TNF-alpha production from human PBMCs. The ED50 for this assay is in the range of 0.125-1.0ug/ml.
Accession No.: P24001
MW: 14900 Da
Sequence:
MCFPKVLSDD MKKLKARMHQ AIERFYDKMQ NAESGRGQVM SSLAELEDDF KEGYLETVAA YYEEQHPELT PLLEKERDGL RCRGNRSPVP DVEDPATEEP GESFCDKSYG APRGDKEELT PQKCSEPQSS K
Purity:
Purity > 97% as determined by reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 10 ug protein lyophilized in 50 mM sodium phosphate pH7.5. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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