IL-6 human active recombinant protein
€295.00
In stock
SKU
600129
Protein Family: Cytokines
Pathway and Disease: Cancers, Immune System, Signal Transduction, Signaling Molecules and Interaction
Alternate Names: interleukin-6, IL-6, B-cell stimulatory factor 2, BSF-2, interferon beta-2, hybridoma growth factor, CTL differentiation factor, CDFDescription:
Cytokines of the IL6/GCSF/MGF family are glycoproteins of about 170 to 180 amino acid residues that contains four conserved cysteine residues involved in two disulphide bonds. Interleukin-6 (IL-6) is an important proinflammatory and immunoregulatory cytokine expressed by various cells. IL-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro. Recombinant Human IL-6 produced in E. coli is a single non-glycosylated polypeptide chain containing 184 amino acids with a MW of 21,000 Da.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
Human IL-6 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of mouse 7TD1 cells is less then 0.08 ng/ml.
Accession No.: P05231
MW: 21000 Da
Sequence:
PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM
Purity:
Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 20 ug protein lyophilized in 10 mM acetic acid. Reconstitute with 0.2 ml sterile 10 mM HCl for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathway and Disease: Cancers, Immune System, Signal Transduction, Signaling Molecules and Interaction
Alternate Names: interleukin-6, IL-6, B-cell stimulatory factor 2, BSF-2, interferon beta-2, hybridoma growth factor, CTL differentiation factor, CDFDescription:
Cytokines of the IL6/GCSF/MGF family are glycoproteins of about 170 to 180 amino acid residues that contains four conserved cysteine residues involved in two disulphide bonds. Interleukin-6 (IL-6) is an important proinflammatory and immunoregulatory cytokine expressed by various cells. IL-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro. Recombinant Human IL-6 produced in E. coli is a single non-glycosylated polypeptide chain containing 184 amino acids with a MW of 21,000 Da.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
Human IL-6 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of mouse 7TD1 cells is less then 0.08 ng/ml.
Accession No.: P05231
MW: 21000 Da
Sequence:
PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM
Purity:
Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 20 ug protein lyophilized in 10 mM acetic acid. Reconstitute with 0.2 ml sterile 10 mM HCl for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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