IL-6 mouse active recombinant protein

IL-6 mouse active recombinant protein

€295.00
In stock
SKU
600192
Catalog Number: 600192
Size: 0.01 mg
Datasheet
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Protein Family: Cytokines

Pathway and Disease: Cancers, Immune System, Signal Transduction, Signaling Molecules and Interaction

Alternate Names: interleukin-6, IL-6, B-cell stimulatory factor 2, Interleukin HP-1, BSF-2, interferon beta-2, B-cell hybridoma growth factor, CTL differentiation factor, CDFDescription:
Interleukin-6 (IL-6), also known as BCDG, BCGF and BSF-2, is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro. Recombinant Mouse IL-6 produced in E. coli is a single non-glycosylated polypeptide chain containing 187 amino acids with a MW of 21,709 Da.

Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Applications: E, WB

Application Notes:
Recombinant mouse IL-6 is fully biologically active when compared to standards. The ED50, as determined by dose-dependent induction of 7TD1 cell proliferation, is .018 ng/ml.

Accession No.: P08505

MW: 21709 Da

Sequence:
MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKELCNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT

Purity:
Purity > 95% as determined by reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and RP-HPLC analysis calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Format:
Each vial contains 10 ug lyophilized protein. Reconstitute with 0.1 ml sterile 10 mM HCl for a final concentration of 0.1 mg/ml.

Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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