Immunoglobulin M (IgM) (class specific) (clone NI 179) FITC, anti-human

Background:
The reactivity of the antiserum is restricted to an isotype specific determinant of the IgM molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunofluorescence staining of cytoplasmic Ig. To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgM and cell membrane IgM. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:50. These data should be interpreted as general recommendations only.

Clone: NI 179

Isotype: Mouse IgG1

Source: Highly purified IgM isolated from pooled human serum

Reactivity: human. The anti-serum does not react with any other component of the human Ig system or any other human plasma protein as tested. This anti-serum has not been tested for crossreactivity with other species.

Specificity: Fluorescein isothiocyanate-conjugated purified monoclonal Mouse antibody to Human IgM, isotype specific.

Form: Purified monoclonal mouse IgG1 kappa conjugated with fluorescein isothiocyanate, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Applications: ELISA, Immunocytochemistry, Immunohistochemistry (frozen), (In)direct immunofluorescence

Storage: Store at 4C, or in small aliquots at -20C.