Inorganic Pyrophosphatase

Inorganic Pyrophosphatase, Recombinant, can be found in many different organisms controlling the cellular concentration of Inorganic Pyrophosphate (PPi) catalyzing its hydrolisis to form orthophophate. This recombinant version is purified from an E. coli strain overexpressing an E. coli gene. This protein is widely used in RNA In Vitro Transcription (IVT) to reduce the inhibitory effects of PPi.

GMP-grade is also available.

Advantages & Features
- Optimized for enhancing DNA replication avoiding the inhibitory effect of PPi and subsequently increasing RNA yield in the In Vitro Transcription reactions.
- Isolated form a recombinant source.
- Extensivelly quality controlled: Endo/exonucleases, and RNAses free.
- High-Purity: ≥ 95% determined by SDS.
- Flexible Quality Systems that covers from RUO to cGMP.

Specifications
Protein information:
- Predicted MW: Homohexamer with 19,71 kDa subunits
- Purity: ≥ 95% by SDS-PAGE
- Buffer: 20 mM Tris-HCl, pH 8.0, 2 mM MgCl2, 2 mM PPi
- Storage: 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH=8
- Biological activity: One unit is the amount of enzyme that will generate 1 µmol ofphosphate per minute from PPi under standard reaction conditions (10 minutes reaction at 25°C) in reaction buffer.

Gene information:
- Synonyms: PPAse
- Official Symbol: E-PPAse
- Specie: Escherichia coli
- GenBank accession number: NC_000913 (4449125-4449652)
- NCBI Protein RefSeq: NP_418647
- Protein Family: Bacterial and archae inorganic pyrophosphatases, hydrolases

Includes
50 U Pyrophosphatase, Inorganic, from E. coli (0,1 U/µL)

Applications
- RNA in vitro transcription (IVT) to reduce the inhibitory effects of PPi.
- mRNA-based Vaccines.
- RNA Labeling.
- Enhancement of DNA replication.

Shipping & Storage
Shipped in: Gel Pack.
Storage: -20 ºC (NON Frost-Free Freezer).