IPTG

IPTG (Isopropyl B-D-thiogalactopyranoside), Dioxane-Free is an analogue of allolactose that binds specifically to the repressor protein of the lac operon and induces expression of β-galactosidase in Escherichia coli. Unlike allolactose, IPTG is not a substrate for b-galactosidase, as the carbon-sulfur bond cannot be cleaved by the enzyme, so its concentration remains constant. The compound is widely used with X-Gal plates to screen for blue/white selection of recombinant bacteria.

Advantages & Features
- Purity: >99.0% confirmed by HPLC.
- Dioxane free: does not disrupt normal cell function.
- High Quality.
- Powder format.

Specifications
- CAS Number: 367-93-1
- Chemical Formula: C9H18O5S
- Molecular Weight: 238.30
- Purity (HPLC)(on dry basis): >99.0%
- Melting point: 110 – 114 °C
- Identity (IR): conforms to structure
- Solubility: soluble in water and methanol
- Heavy metals (Pb): <5ppm
- 1,4-Dioxane: Not detected
- pH (5% in water): 5.0 – 7.0
- Water content (Karl Fischer): <1.0%

Includes
25 g IPTG

Applications
- Blue/white screening.
- Expression of genes under lac promoter control.

Shipping & Storage
Shipped in: Gel pack.
Storage: 4 ºC .