Keratin 13 + 10 (clone EBS-IF-003), anti-human, cat, dog

Source:
A hybrid (H2d) mouse was immunized with a cytoskeleton preparation from HeLa cells.
Fusion partner: Sp2/0.

Specifications:
EBS-IF-003 reacts with 53kDa (CK13) and 56.6kDa (CK10) cytokeratin proteins as indicated by immunoblotting. On formalin-fixed, paraffin-embedded tissue sections EBS-IF-003 recognizes only CK13. On cryostat sections EBS-IF-003 serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas. In paraffin sections EBS-IF-003 does not stain CK10 positive, CK13 negative epithelia, as for example epidermis.

Species reactivity:
Positive: cat, dog, human.

Applications:
Demonstration of keratin 13 in immunohistochemistry, immunofluorescence tests and immunoblotting.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: CK13 (citrate)
Western blot: +

Positive control: A431, HeLa, MCF7 cells. Tonsil, esophageal or bladder carcinoma.

References:
- Ivanyi D. et al, J. Pathol. 159: 7-12 (1989).
- Ivanyi D. et al, Am. J. Vet. Res. 53: 304-314 (1992).
- Ivanyi D. et al, Am. J. Vet. Res. 54: 1095-1102 (1993).
- Kozaki, M et al, J. Vet. Med. Sci.63(1): 1-4 (2001)