Keratin 18 (clone C-04), anti-human, mouse, rat, bovine, cat, dog, ferret, goat, hamster, horse, marmoset, pig, sheep

Source:
A hybrid (BALB/c x B6) mouse was immunized with a cytoskeleton preparation of human A431 carcinoma cell line.
Fusion partner: Sp2/0.

Specifications:
C-04 Reacts with keratin 18 (45kDa) present in a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.

Species reactivity:
Positive: cat, cow, dog, ferret, goat, hamster, horse, human, marmoset, mouse, pig, rat,sheep.

Applications:
Immunohistochemistry on frozen and paraffin sections. Dominant positive structures include a wide variety of simple epithelia, urinary bladder transitional epithelia. Positive in fluorescence tests and immunoblotting.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Pronase
Western blot: +

Positive control: MCF-7, HeLa cells, breast cancer.

References:
- Bártek et al. J. of Pathol.164: 215-24 (1991).
- Bártková et al. Neoplasma38(4): 439-46 (1991).
- Lauerová et al. Hybridoma7: 495-504 (1988).
- Kovařík et al. Int. J. CancerSuppl. 3: 50-5 (1988).
- Taylor –Papadimitriou et.al. J. Cell Sci.94: 403-13 (1989).
- Vojtĕšek et al. Folia Biol.35(6): 373-382 (1989).
- Kovařík et al. J. Tumor Marker Oncol. 5: 219 (1990).