Keratin 18 (clone DC10), anti-human

Source:
A hybrid mouse (BALB/c x B6) was immunized with a PMC-42 human breast carcinoma cell line.
Fusion partner: SP2/0.

Specifications:
DC10 reacts with human keratin 18 (45kD), found in a wide variety of simple epithelia. It does not react with stratified squamous epithelia. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. Gastrointestinal tract tissues are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Furthermore, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for keratin 18.

Species reactivity:
Positive: human. Negative: cow, dog, ferret, hamster, mouse, pig, rat, sheep.

Applications:
Keratin 18 antibodies reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate
Western blot: +

Positive control: MCF-7, HeLa cells, Breast Cancer.

References:
- Lauerová, L Hybridoma, 7, 495-504 (1988).
- Kovařík et al. Int.J.Cancer, Suppl. 3: 50-55, (1988).
- Vojtĕsek, B. et al. Folia Biol., 35(6), 373-382, 1989
- Kovařík et al. J. Tumor Marker Oncol.5, 219 (1990).
- Michal, M. Histochemical J., 22, 170 (1990)