Keratin 5 + 14 (clone EBS-IF-001), anti-human, cat

Source:
A hybrid (BALB/c x B6) mouse was immunized with a cytokeratin-enriched extract of feline tongue epithelium.
Fusion partner: Sp2/0.

Specifications:
EBS-IF-001 Does not react with keratin polypeptides in immunoblotting. The specificity for keratin was established on the basis of antibody reactivity with intracytoplasmic intermediate filaments in epithelial, vimentin negative, human and feline cells. Distribution of the epitope strongly suggests that EBS-IF-001 reacts with keratins 5 and 14, most probably with a heterotypic complex formed by these keratins. EBS-IF-001 reacts positively with basal cells of all stratified epithelia, with myoepithelial cells and with most squamous cell carcinomas. It is useful in immunohistochemistry for typing of basal cells.

Species reactivity:
Positive: cat, human.

Applications:
Demonstration of keratin 5 and 14 in immunohistochemistry on frozen sections and immunofluorescence tests.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin section: -
Western blo: +

Positive control: Tongue.

References:
- Ivanyi et al. Am. J. Vet. Res. 53: 304-314 (1992).
- Balm A.J.M., Eur. Arch. Otorhinolaryngol.253: 227-233 (1996).