Keratin 8 + 18 (clone C-51), anti-human, bovine, monkey, pig, sheep

Source:
A BALB/c mouse was immunized with a cytoskeleton preparation from HeLa cells.
Fusion partner: Sp2/0.

Specifications:
C-51 reacts with keratin 8 (52.5 kDa) + 18 (45 kDa) polypeptides and recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas arepositive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.

Species reactivity:
Positive: cow, human, monkey, pig, sheep. Negative: chicken, dog, ferret, hamster, mouse,Xenopus laevis.

Applications:
C-51 can be used on paraffin sections using either citrate or protease pre-treatment.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate/protease
Western blot: +

Positive control: MCF-7 or A431 cells. Skin, colon, lung or breast carcinoma.

References:
- Bártek et al. J. Pathol.164: 215-24 (1991).
- Bártková et al. Neoplasma38(4): 439-46 (1991).
- Wendl, J. et al. Anat. Histol Embryol.164: 215-24 (1991).
- Thangappan, R et al. Cell. Polif.42: 770-779 (2009).
- Imbalzano, KL, et al. PLoS.One.8: e55628 (2013)