KlenTaq LA

KlenTaq LA

€0.00
In stock
SKU
VN110E
Catalog Number: VN110E
Size: 100 µl (1000~2000 x 25 µl rxns)
Other Size(s): 50 µl (500~1000 x 25 µl rxns)
Datasheet
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Inhibition-resistant Taq Pols & Master Mixes (17)

- Inhibition-Resistant Taq DNA Polymerases are obtained by molecular engineering through mutagenesis of DNA polymerase gene to render enzymes resistant to PCR inhibitors such as blood, plasma, serum, soil, plant, water, chocolate, cheese and milk.
- Proven applications include forensics, environmental testing, agriculture and clinical diagnostics.
- Up to 40% whole blood, plasma or serum are tolerated and varying amounts of soil, plant and inhibitory foods.
- Provides superior amplification, even on damaged or degraded DNA templates.
- DNA extraction is often unnecessary, saving time and enabling amplification from minute quantities of DNA.
- Can tolerate more PCR inhibitors when combining with PCR enhancers.

KlenTaq DNA Polymerase is a Taq polymerase variant that lacks the 5’-exonuclease activity due to an N-terminal deletion of Taq. This modification results in improved fidelity and thermostability compared to the wild-type Taq. Although not as strong as our new generation of KlenTaq or Taq mutants, KlenTaq does have some inhibition-resistant features.

KlenTaq DNA Polymerase is suitable for real-time PCR with DNA binding dyes, such as SYBR Green and Eva Green. However, it cannot be used in real-time PCR TaqMan assays, which require 5’-exonuclease activity. This enzyme is inhibition-resistant as well and able to tolerate up 30% blood in PCR.

KlenTaq LA DNA Polymerase is the Long Accurate version of KlenTaq, a Taq polymerase variant that lacks 5’-exonuclease activity due to an N-terminal deletion of Taq. This modification results in improved fidelity and thermostability compared to the wild-type Taq. KlenTaq LA is designed to amplify longer gene targets compared to the standard KlenTaq polymerase.

KlenTaq LA DNA Polymerase can be used in real-time PCR with DNA binding dyes, such as SYBR Green and Eva Green. However, it is not suitable for real-time PCR TaqMan assays that require 5’-exonuclease activity.

Highlights

- Thermostability - more thermostable and improved fidelity than Taq DNA polymerase
- Efficient – novel buffer system maximizes efficiency of PCR amplification, increases yield of any PCR product
- Robust – reliable amplification in the presence of inhibitors and with even the most challenging DNA targets
- Flexible – LA version is ideal for amplifying any target up to 30 kb from DNA extracted from human, animal and plant samples
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