KlenTaq-P

KlenTaq-P is a 5’-exonuclease deficient Taq polymerase (an N-terminal deletion of Taq) with improved fidelity and thermostability, plus an extra mutation rendering the enzyme PAP function (Pyrophosphorolysis-activated polymerization). The enzyme is capable of detecting rare mutations, large heterozygous deletions, gene duplications, etc. in the presence of a large excess of wild type allele and inorganic pyrophosphate. In theory PAP can detect a single base mutation in 3x1011copies of wild type allele.

KlenTaq-P LA is a blending of KlenTaq-P and a proofreading enzyme.

Applications:

Rare mutations detection Large heterozygous deletions