KlenTaq-S LA
€0.00
In stock
SKU
VN110ES
Catalog Number: VN110ES
Size: 100 µl (1000~2000 x 25 µl rxns)
Other Size(s): 50 µl (500~1000 x 25 µl rxns)
Datasheet
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Size: 100 µl (1000~2000 x 25 µl rxns)
Other Size(s): 50 µl (500~1000 x 25 µl rxns)
Datasheet
Request Information
Taq & KlenTaq Pols and Master Mixes (6)
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´→3 DNA polymerase activity and a 5´→3´ exonuclease activity. Recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli.
KlenTaq® is a Klenow-fragment analog of Taq DNA polymerase. It is a 5’-exonuclease deficient Taq polymerase (an N-terminal deletion of Taq) with improved fidelity, robust and thermostability.
KlenTaq-S is a 5’-exonuclease deficient Taq polymerase (an N-terminal deletion of Taq) with improved fidelity and thermostability, plus an extra mutation rendering the enzyme PAP function (Pyrophosphorolysis-activated polymerization). The enzyme is capable of detecting rare mutations, large heterozygous deletions, gene duplications, etc. in the presence of a large excess of wild type allele and inorganic pyrophosphate). In theory PAP can detect a single base mutation in 3x1011copies of wild type allele.
Taq and Klentaq LA® (Long Accurate) versions of the enzymes are mixtures of Taq / KlenTaq and a proof reading enzyme. They can be used to amplify a long gene target with high fidelity.
KlenTaq-S DNA Polymerase is a 5’-exonuclease deficient Taq polymerase (an N-terminal deletion of Taq) with improved fidelity and thermostability, as well as an extra mutation providing the enzyme with PAP function (Pyrophosphorolysis-activated polymerization). The enzyme is capable of detecting rare mutations, large heterozygous deletions, gene duplications, and more, even in the presence of a large excess of wild-type allele and inorganic pyrophosphate. In theory, PAP can detect a single base mutation in 3x10^11 copies of wild-type allele.
This enzyme can be used in real-time PCR with DNA binding dyes, such as SYBR Green and Eva Green, but it cannot be used in real-time PCR TaqMan assay, which requires 5’-exonuclease activity. If a TaqMan assay is required, it can be blended with our full-length Taq mutant enzymes.
KlenTaq-S LA DNA Polymerase is a Long and Accurate version of KlenTaq-S, which is a Taq polymerase with an N-terminal deletion that makes it 5’-exonuclease deficient. KlenTaq-S LA DNA Polymerase has enhanced fidelity and thermostability, as well as an additional mutation that enables it to perform pyrophosphorolysis-activated polymerization (PAP) function. These properties make it suitable for amplifying longer gene targets and detecting rare mutations, large heterozygous deletions, and gene duplications even in the presence of a large excess of wild-type alleles and inorganic pyrophosphate.
KlenTaq-S LA DNA Polymerase can be used in real-time PCR with DNA binding dyes, such as SYBR Green and Eva Green, but it cannot be used in real-time PCR TaqMan assays that require 5’-exonuclease activity. However, it can be blended with full-length Taq mutant enzymes for TaqMan assays.
Application:
- Rare mutations detection
- Large heterozygous deletions
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´→3 DNA polymerase activity and a 5´→3´ exonuclease activity. Recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli.
KlenTaq® is a Klenow-fragment analog of Taq DNA polymerase. It is a 5’-exonuclease deficient Taq polymerase (an N-terminal deletion of Taq) with improved fidelity, robust and thermostability.
KlenTaq-S is a 5’-exonuclease deficient Taq polymerase (an N-terminal deletion of Taq) with improved fidelity and thermostability, plus an extra mutation rendering the enzyme PAP function (Pyrophosphorolysis-activated polymerization). The enzyme is capable of detecting rare mutations, large heterozygous deletions, gene duplications, etc. in the presence of a large excess of wild type allele and inorganic pyrophosphate). In theory PAP can detect a single base mutation in 3x1011copies of wild type allele.
Taq and Klentaq LA® (Long Accurate) versions of the enzymes are mixtures of Taq / KlenTaq and a proof reading enzyme. They can be used to amplify a long gene target with high fidelity.
KlenTaq-S DNA Polymerase is a 5’-exonuclease deficient Taq polymerase (an N-terminal deletion of Taq) with improved fidelity and thermostability, as well as an extra mutation providing the enzyme with PAP function (Pyrophosphorolysis-activated polymerization). The enzyme is capable of detecting rare mutations, large heterozygous deletions, gene duplications, and more, even in the presence of a large excess of wild-type allele and inorganic pyrophosphate. In theory, PAP can detect a single base mutation in 3x10^11 copies of wild-type allele.
This enzyme can be used in real-time PCR with DNA binding dyes, such as SYBR Green and Eva Green, but it cannot be used in real-time PCR TaqMan assay, which requires 5’-exonuclease activity. If a TaqMan assay is required, it can be blended with our full-length Taq mutant enzymes.
KlenTaq-S LA DNA Polymerase is a Long and Accurate version of KlenTaq-S, which is a Taq polymerase with an N-terminal deletion that makes it 5’-exonuclease deficient. KlenTaq-S LA DNA Polymerase has enhanced fidelity and thermostability, as well as an additional mutation that enables it to perform pyrophosphorolysis-activated polymerization (PAP) function. These properties make it suitable for amplifying longer gene targets and detecting rare mutations, large heterozygous deletions, and gene duplications even in the presence of a large excess of wild-type alleles and inorganic pyrophosphate.
KlenTaq-S LA DNA Polymerase can be used in real-time PCR with DNA binding dyes, such as SYBR Green and Eva Green, but it cannot be used in real-time PCR TaqMan assays that require 5’-exonuclease activity. However, it can be blended with full-length Taq mutant enzymes for TaqMan assays.
Application:
- Rare mutations detection
- Large heterozygous deletions
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