(KO Validated) APEX1 Polyclonal Antibody

Background:
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein.

Research Area: Cancer, Cardiovascular, Epigenetics and Nuclear Signaling

Synonyms:
APEX1, APE, APE1, APEN, APEX, APX, HAP1, REF1

Immunogen: Recombinant fusion protein of human APEX1 (NP_542380.1).

Swissprot: P27695
Gene ID: 328

Calculated MW: 35 kDa
Observed MW: 39 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Concentration: 1 mg/mL
Dilution: "WB 1:500-1:2000 IF 1:50-1:100"

Storage:
Store at -20°C. Avoid freeze / thaw cycles.