(KO Validated) Vimentin Polyclonal Antibody

Background:
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.

Research Area: Cancer, Developmental biology, Neuroscience, Signaling transduction, Stem cells

Synonyms:
CTRCT30, HEL113, Vimentin, VIM, vimentin

Immunogen: Recombinant fusion protein of human Vimentin (NP_003371.2).

Swissprot: P08670
Gene ID: 7431

Calculated MW: 53 kDa
Observed MW: 57 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Concentration: 1 mg/mL
Dilution: "WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200"

Storage:
Store at -20°C. Avoid freeze / thaw cycles.