L-Histidine Separopore® 6B-CL

Application: Histidine-Separopore® 6B-CL can be used in the purification of supercoiled (sc) plasmid DNA (pDNA) from Escherichia coli lysates. This can also be used for the separation of IgG from human plasma and selective purification of bioactive oligouronides such as α-(1, 4)-oligogalacturonides. It is used for the purification of histidine binding proteins. This matrix can also be used for the purification of proteins expressed in Escherichia coli that use the coordination of a heme tag. This approach provides an affinity purification tag visible to the eye, facilitating tracking of the protein.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Technical Specifications:
Ligand: L-Histidine
Matrix: Separopore® 6B-CL (crosslinked agarose beads, 6%)
Particle size range: 52 – 180 µm
Molecular weight range: 6 x 104 – 2 x 107
Matrix active group: Epoxy
Matrix spacer: 12 atoms
Attachment: Amino
Extent of coupling: 1 – 2 μmol / ml
pH stability: 4 – 9
Flow Specifications: 70 – 140 cm / h
Supplied as a suspension in 2M NaCl, 0.02% thimerosal
Storage: 2 – 8 °C

References:
el-Kak A, Vijayalakshmi MA. Separation and purification of IgG1 and IgG2 from IgG-Cohn-II fraction of human plasma by pseudo-bioaffinity chromatography on histidyl-AH-sepharose. Bioseparation. 1992; 3(1):47-53. PMID: 1369217.
Delattre C, Kamalanathan AS, Michaud P, Vijayalakshmi MA. Purification of oligouronides by immobilized L-histidine pseudoaffinity chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Jan 15; 861(2):181-5. PMID: 17625991.
Asher WB, Bren KL. A heme fusion tag for protein affinity purification and quantification. Protein Sci. 2010 Oct; 19(10):1830-9. PMID: 20665691; PMC2998719.
Braun M, Rubio IG, Thöny-Meyer L. A heme tag for in vivo synthesis of artificial cytochromes. Appl Microbiol Biotechnol. 2005 Apr; 67(2):234-9. PMID: 15834717.

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Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Hazmat Shipping: Non-Hazardous
Storage: 2-8°C