Lactate Dehydrogenase (LDH) Activity Assay Kit

Detection Principle:
Using coenzyme I as a hydrogen carrier, LDH catalyze lactic acid to produce pyruvate. Pyruvate reacted with 2, 4-dinitrophenylhydrazine to form pyruvate dinitrophenylhydrazone, which was red-brown in alkaline solution, and the color depth was proportional to pyruvate concentration. The activity of LDH could be calculated by measuring OD value.
Instrument: Microplate reader
Assay Time: 45 min

Tested Sample Types: Serum,plasma,hydrothorax,tissue,cells

Research Area's: Enzymes,Glycolysis & Carbohydrates,Liver Biomarkers

Shelf Life: 6 Months

Storage:
2-8℃

Citations:


Detection Principle: https://www.spandidos-publications.com/10.3892/ijmm.2019.4123
https://onlinelibrary.wiley.com/doi/abs/10.1002/jbt.22283
https://pubmed.ncbi.nlm.nih.gov/32643556/
https://pubmed.ncbi.nlm.nih.gov/32978744/
https://pubmed.ncbi.nlm.nih.gov/32871779/