Lassa virus (LASV)-NP (clone EBS-I-315), anti-human, other animals

Source:
A BALB/c mouse was immunized with gamma rays inactivated strain LASV.
Fusion partner: Sp2/0.

Specifications:
Lassa virus is a member of the Arenaviridae and causes Lassa fever in predominantly West Afrika. The main reservoir is formed by local rodents. Up to half a million people are estimated to attract the disease yearly and mortality rates may reach as much as 50%. Viral proteins, coded within two ambisense RNA strands, include GP1, GP2, NP, polymerase and Z matrix protein. EBS-I-315 reacts with NP, which harbor the RNA strands within the virion. Reactivity of EBS-I-315 includes isolates from Sierra Leone, Guinea, Liberia, Nigeria and Southern African countries. However, isolates from the Central AfricanRepublic were all negative. The epitope is different from the epitope recognized by the NP antibody EBS-I-314.

Species reactivity:
Positive: (humanand animals subject to infection with)Lassa virus.

Applications:
EBS-I-315 is excellent for immunohistology, immunofluorescence, immunoblotting, immunoprecipitation and ELISA.
ELISA: +
Frozen sections: +
Immunofluorescence: +
Lateral flow: +
Western blot: +

Positive control: Cells, serum or tissues infected with Lassa virus.

References:
- Branco L.M. et al. Virol. J.7:279-298 (2010).
- Ruo S.L et al., J. Gen. Virol.72, 549-555 (1991).