M-MLV Reverse Transcriptase

M-MLV Reverse Transcriptase (MMLV-RT), encoded by Moloney Murine Leukemia Virus (MMLV) is an RNA dependent DNA polymerase that synthesizes the cDNA first strand from a single-stranded RNA template to which a primer has been hybridized. MMLV-RT will also extend primers hybridized to single-stranded DNA.

Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase.

Source: purified from E.coli strain harboring a plasmid that directs the synthesis of modified form of MMLV-RT.

MW: 69 KDa.

GMP-grade reagent also available.



Advantages & Features
- Concentration: 200 U/µL.
- Full Solution: includes Reaction Buffer 5x.
- High Quality: free of DNAse, RNase or protease contamination.
- Purity: >90% as judged by SDS-polyacrylamide gels with blue staining.
- Final Product Size: 7 kb or less.

Specifications
Unit definition:
One unit of the enzyme incorporates 1 nmol dTTP into acid-precipitable material in 10 minutes at 37 ° C, using poly(A) oligo dT as a template primer.

Includes
Includes:
- M-MLV Reverse Transcriptase (200 U/μL)
- Reaction Buffer (10x)

Applications
- RT PCR.
- Synthesis of cDNA.
- mRNA 5’-end Mapping by Primer Extension Analysis.
- End-labeling of DNA.
- Dideoxynucleotide Sequencing.

Shipping & Storage
Shipped in: Gel pack.
Storage: -20 ºC (NON Frost-Free Freezer).