Maackia amurensis Lectin (MAA/MAL I) - Alexa Fluor 647
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SKU
BW-21511525
Description
Maackia amurensis Lectin (MAA/MAL I) is a leukoagglutinating lectin isolated from Maackia amurensis seeds and purified by affinity chromatography. MAA I can be used to detect N-linked glycans containing the trisaccharide Siaα2-3Galß1-4GlcNAc. This lectin does not agglutinate one blood type more than another and agglutination can be inhibited by sialyllactose or lactose at higher concentrations. Although MAA I and MAA II are similar in size and structure, this lectin is a more potent mitogen and exhibits less hemagglutinating ability than MAA II.
This lectin is used in a plethora of research. It has been exhibited that MAA inhibits oral squamous cell carcinoma growth and motility by decreasing activity of the JAK-STAT, TGFβ-SMAD, and Wnt-βCTN signaling pathways. MAA has been used to study Turkey adenovirus 3 activity, leaving evidence that the virus has attachment receptors that are both α2,3-linked and α2,6-linked sialic acids. As a sialic acid binding lectin, MAA has been used to observe content of sialic acid in seminoma testicular tissue, revealing that sialic acids may have roles in seminoma development by promoting invasiveness, as well as cross-talk between cancer-cells and the surrounding stroma and vessels. In novel SARS-CoV-2 research, it has been shown that MAA targets the ACE2 receptor, thus decreasing glycosylation and expression. As a result, the spike protein binding is suppressed, and inflammatory mediators have decreased expression.
When bound to Alexa Fluor 647, the binding pattern of MAA I in cellular imaging and flow cytometry is shown. The excitation wavelength required for Alexa Fluor 647 to fluoresce is high enough to avoid overlap with most other fluorochromes, making it useful for dual-labeling experiments. Because of this high excitation, there is typically less background from autofluorescence of biological specimens. This product comes in a stabilized liquid form.
bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical
Specifications:
Source: Maackia amurensis (Amur maackia)
Activity: Non-specific
Carbohydrate Specificity: Siaα2-3Galß1-4GlcNAc
Molecular Weight: 130 kDa
Inhibitory Carbohydrate: Lactose
Divalent Ions Required: None
Mitogenic Activity: Yes
Conjugation: Alexa Fluor 647
Excitation: Maximum at 651 nm
Emission: Maximum at 672 nm
Storage and Stability:
Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8°C for short term use.
Application
Blotting, Glycobiology, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Mitogenic Stimulation
References
Hamilton, K. L., Sheehan, S. A., Retzbach, E. P., Timmerman, C. A., Gianneschi, G. B., Tempera, P. J., Balachandran, P., & Goldberg, G. S. (2021). Effects of Maackia amurensis seed lectin (MASL) on oral squamous cell carcinoma (OSCC) gene expression and transcriptional signaling pathways. Journal of cancer research and clinical oncology, 147(2), 445–457.
https://doi.org/10.1007/s00432-020-03456-8
Mahsoub, H. M., Yuan, L., & Pierson, F. W. (2020). Turkey adenovirus 3, a siadenovirus, uses sialic acid on N-linked glycoproteins as a cellular receptor. The Journal of general virology, 101(7), 760–771. https://doi.org/10.1099/jgv.0.001429
Marini, M., Tani, A., Manetti, M., & Sgambati, E. (2020). Characterization and distribution of sialic acids in human testicular seminoma. Acta histochemica, 122(3), 151532. https://doi.org/10.1016/j.acthis.2020.151532
Sheehan, S. A., Hamilton, K. L., Retzbach, E. P., Balachandran, P., Krishnan, H., Leone, P., Lopez-Gonzalez, M., Suryavanshi, S., Kumar, P., Russo, R., & Goldberg, G. S. (2021). Evidence that Maackia amurensis seed lectin (MASL) exerts pleiotropic actions on oral squamous cells with potential to inhibit SARS-CoV-2 infection and COVID-19 disease progression. Experimental cell research, 403(1), 112594. https://doi.org/10.1016/j.yexcr.2021.112594
Properties
Storage Temperature: -20°C
Hazmat Ship: Non-hazardous
Maackia amurensis Lectin (MAA/MAL I) is a leukoagglutinating lectin isolated from Maackia amurensis seeds and purified by affinity chromatography. MAA I can be used to detect N-linked glycans containing the trisaccharide Siaα2-3Galß1-4GlcNAc. This lectin does not agglutinate one blood type more than another and agglutination can be inhibited by sialyllactose or lactose at higher concentrations. Although MAA I and MAA II are similar in size and structure, this lectin is a more potent mitogen and exhibits less hemagglutinating ability than MAA II.
This lectin is used in a plethora of research. It has been exhibited that MAA inhibits oral squamous cell carcinoma growth and motility by decreasing activity of the JAK-STAT, TGFβ-SMAD, and Wnt-βCTN signaling pathways. MAA has been used to study Turkey adenovirus 3 activity, leaving evidence that the virus has attachment receptors that are both α2,3-linked and α2,6-linked sialic acids. As a sialic acid binding lectin, MAA has been used to observe content of sialic acid in seminoma testicular tissue, revealing that sialic acids may have roles in seminoma development by promoting invasiveness, as well as cross-talk between cancer-cells and the surrounding stroma and vessels. In novel SARS-CoV-2 research, it has been shown that MAA targets the ACE2 receptor, thus decreasing glycosylation and expression. As a result, the spike protein binding is suppressed, and inflammatory mediators have decreased expression.
When bound to Alexa Fluor 647, the binding pattern of MAA I in cellular imaging and flow cytometry is shown. The excitation wavelength required for Alexa Fluor 647 to fluoresce is high enough to avoid overlap with most other fluorochromes, making it useful for dual-labeling experiments. Because of this high excitation, there is typically less background from autofluorescence of biological specimens. This product comes in a stabilized liquid form.
bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical
Specifications:
Source: Maackia amurensis (Amur maackia)
Activity: Non-specific
Carbohydrate Specificity: Siaα2-3Galß1-4GlcNAc
Molecular Weight: 130 kDa
Inhibitory Carbohydrate: Lactose
Divalent Ions Required: None
Mitogenic Activity: Yes
Conjugation: Alexa Fluor 647
Excitation: Maximum at 651 nm
Emission: Maximum at 672 nm
Storage and Stability:
Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8°C for short term use.
Application
Blotting, Glycobiology, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Mitogenic Stimulation
References
Hamilton, K. L., Sheehan, S. A., Retzbach, E. P., Timmerman, C. A., Gianneschi, G. B., Tempera, P. J., Balachandran, P., & Goldberg, G. S. (2021). Effects of Maackia amurensis seed lectin (MASL) on oral squamous cell carcinoma (OSCC) gene expression and transcriptional signaling pathways. Journal of cancer research and clinical oncology, 147(2), 445–457.
https://doi.org/10.1007/s00432-020-03456-8
Mahsoub, H. M., Yuan, L., & Pierson, F. W. (2020). Turkey adenovirus 3, a siadenovirus, uses sialic acid on N-linked glycoproteins as a cellular receptor. The Journal of general virology, 101(7), 760–771. https://doi.org/10.1099/jgv.0.001429
Marini, M., Tani, A., Manetti, M., & Sgambati, E. (2020). Characterization and distribution of sialic acids in human testicular seminoma. Acta histochemica, 122(3), 151532. https://doi.org/10.1016/j.acthis.2020.151532
Sheehan, S. A., Hamilton, K. L., Retzbach, E. P., Balachandran, P., Krishnan, H., Leone, P., Lopez-Gonzalez, M., Suryavanshi, S., Kumar, P., Russo, R., & Goldberg, G. S. (2021). Evidence that Maackia amurensis seed lectin (MASL) exerts pleiotropic actions on oral squamous cells with potential to inhibit SARS-CoV-2 infection and COVID-19 disease progression. Experimental cell research, 403(1), 112594. https://doi.org/10.1016/j.yexcr.2021.112594
Properties
Storage Temperature: -20°C
Hazmat Ship: Non-hazardous
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