MEM, NEAA (with glucose, Earle's salts, without glutamine, phenol red, sodium pyruvate)

Backgroud: MEM medium (Minimum Essential Medium) is also called the minimum essential medium, minimum basic medium or low-limit Eagle medium. It was developed by Harry Eagle based on Eagle Basic Medium (BEM). It is the most basic and widely applicable medium and one of the most commonly used mediums in animal cell culture. MEM medium contains only 12 essential amino acids, glutamine and 8 vitamins. It has simple ingredients and is mainly used for the culture of adherent cells. It can also be used for other types of cell culture after the formula is modified. MEM medium containing NEAA (non-essential amino acids) is based on MEM medium and adds 7 NEAAs: L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline, L-serine and glycine. It can reduce the side effects of the production of non-essential amino acids by cells themselves during cell culture and effectively promote cell proliferation and metabolism.


L-glutamine is a necessary nutrient element in cell culture, but it is unstable in solution and will degrade spontaneously. The medium without L-glutamine can adjust the amount of L-glutamine according to research needs. Adding fresh L-glutamine or its substitute during use is more conducive to cell growth.

Contains: L-Glutamine: Without, NaHCO3Without, D-Glucose1g/L, Sodium PyruvateWithout, HEPESWithout, Phenol RedWithout, Balanced Salt SolutionEarle's, NucleosideWithout, NEAAWith

Shipping: Room temperature

Storage: Store at 2-8°C away from light

Shelf Life: 24 months