MHC I A (clone 108-2C5), anti-human

Source:
A BALB/c mouse was immunized with human PBL.
Fusion partner: NS-1.

Specifications:
108-2C5 recognizes an intralocus determinant present on a limited number of HLA-A locus-encoded gene products (HLA-A2, -A3, A28, -A29, -A30, -A31 and -Aw33). Furthermore, by testing its reactivity with HLA-A2 natural variants and mutants, the importance of amino acid residues 79 and/or 80 of the alpha1 domain was demonstrated in the formation of an intralocus HLA-A determinant.

Species reactivity:
Positive: human.

Applications:
108-2C5 is useful for HLA-A typing of human PBL, leukemia’s and leukemic cell lines.
Flow cytometry: +
Frozen sections: +

Positive control: THP-1 and Jurkat cells. Tonsil or lymph node.

References:
- Lozano, F. et al., Immunogenetics30: 50-53 (1989).
- Lozano, F. et al., Tissue Antigens35: 193-195 (1990).
- Domenech, N. et al., Human Immunol30: 140-146 (1991).
- Ryschich, E, et al, Clin Cancer Res.11(2 Pt 1): 498-504 (2005)