MHC I B27 (clone EP-4), anti-human

Source:
A BALB/c mouse was immunized with lymphocytes from an HLA-B27 patient.
Fusion partner: NS-1.

Specifications:
EP-4 recognizes the HLA-B27 cell surface antigen on human cells. HLA-B27 has been found to be highly associated (60%) with ankylosing spondylitis (Bekhterev's disease, Marie-Strümpell disease or "bamboo spine"). While rheumatoid factor tests will be negative, testing for the presence of HLA-B27 in a patient can confirm the diagnosis of ankylosing spondylitis. Also postgonococcal arthritis and acute anterior uveitis are associated with HLA-B27.

Species reactivity:
Positive: human.

Applications:
EP-4 is excellent for demonstration of HLA- B27.
Frozen sections: +
Flow cytometry: +
Immunofluorescence: +

Positive control: Human peripheral blood lymphocytes, tonsil or lymph node.

References:
- Hansen JA et al. Hematol Oncol Clin North Am,4(3): 507-515 (1990).
- El-Shabrawi, Y. et al. Ophthalmology113: 695-700 (2006).
- Kellner H, Clin Investig.72(4): 321-8 (1994).