MHC I Betha2-microglobulin (clone EBS-O-109), anti-human

Source:
A BALB/c mouse was immunized with human PBL from a T-ALL patient.
Fusion partner: NS-1.

Specifications:
EBS-O-109 reacts with human β-2 microglobulin, a 22 kDa protein, which associates non-covalently with the 44kDa α1-chain of the HLA Class I complex found on all nucleated cells and on platelets. There is no reaction with erythrocytes, neither with non-human primate cells. The detection of β-2 microglobulin in body fluids has been used as a tumor marker, renal failure marker and for monitoring patients with HIV infection.

Species reactivity:
Positive: human. Negative: non human primates.

Applications:
Detection of human β-2 microglobulinas a.o.tumor marker, renal failure marker and for monitoring HIV patients.
ELISA: +
Flow cytometry: +
Frozen sections: +

Positive control: Human PBL.

References:
- Brodsky FM et al, lmmunol Rev47: 3-61 (1979).
- Sparrow R.L. Human cell surface antigens defined by monoclonal antibodies. PhD thesis, University of Melbourne, (1983).
- Ryschich, E, et al, Clin Cancer Res.11(2 Pt 1): 498-504 (2005).
- Betts RL, et al. Monoclonal hybridoma antibodies: Techniques and applications. Edited by D. Hurrel. Uniscience series program. C.R.C. Press, Cleveland, OH: 1983, pp. 193-222
- Cosgrove LJ, et al, Immunol. Cell Biol. 66 (1): 69-77 (1988).