MHC II DP (betha chain) (clone BraFB6), anti-human

Source:
A BALB/c mouse was immunized with non-T, non-B human acute lymphoblastic leukemia REH6 cells.
Fusion partner: NS-1.

Specifications:
MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an αand βchain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.

Species reactivity:
Positive: human.

Applications:
Demonstration of MHC II DRA.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate

Positive control: Raji cells. Tonsil or lymph node.

References:
- Babusikova O et al., Neoplasma32(6): 657-62 (1985).
- Polakova K et al., Neoplasma, 32(6): 641-8 (1985).
- Horejsi V, et. al. Tissue Antigens, 32(1): 6-11 (1988)