MHC II DP+DR (beta chain) (clone Bra14), anti-human

Source:
A BALB/c mouse was immunized with nuclei from pokeweed mitogen-stimulated PBL.
Fusion partner: NS-1.

Specifications:
MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an αand βchain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.

Species reactivity:
Positive: human.

Applications:
Demonstration of MHC II DP and DR beta chain.
Flow cytometry: +
Frozen sections: +
Paraffin sections: +

Positive control: Ramos, Daudi or HuT78 cells. Tonsil or lymph node.

References:
- Chorvath B et al. Neoplasma34(4):417-425 (1987).
- Horejsi V et al. Tissue Antigens32(1):6-11 (1988).
- Polakova K et al. Neoplasma 32(6):641-8 (1985).