MHC II DR (alpha, betha chain) (clone Bra30), anti-human

Source:
A BALB/c mouse was immunized with REH (human ALL cell line).
Fusion partner: SP-2/0.

Specifications:
MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an αand βchain.Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.

Species reactivity:
Positive: human.

Applications:
Demonstration of MHC II DR beta chain.
Flow cytometry: +
Frozen section: +
Paraffin sections: Citrate

Positive control: Ramos, Daudi or HuT78 cells. Tonsil or lymph node.

References:
- Chorvath B et al. Neoplasma34(4): 417-425 (1987).
- Horejsi V et al. Tissue Antigens32(1): 6-11 (1988).
- Polakova K et al. Neoplasma 32(6): 641-8 (1985)