Monkey Ang-Ⅱ (Angiotensin Ⅱ) ELISA Kit

Description:
This ELISA kit applies to the in vitro quantitative determination of Monkey Ang-Ⅱ concentrations in serum, plasma and other biological fluids.

Assay Principles: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated wit Monkey Ang-Ⅱ. During the reaction, Monkey Ang-Ⅱ in the sample or standard competes with a fixed amount of Monkey Ang-Ⅱ on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Monkey Ang-Ⅱ. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Monkey Ang-Ⅱ in the samples is then determined by comparing the OD of the samples to the standard curve.

Species reactivity:
Monkey

Sample Type: serum, plasma, cell lysates, tissue homogenates, cell culture supernatant or other biological fluids

Storage instruction: -20˚C and 4˚C for 6 months