Monkey RANκL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit

Description:
This ELISA kit applies to the in vitro quantitative determination of Monkey RANκL concentrations in serum, plasma and other biological fluids.

Assay Principles: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey RANκL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey RANκL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey RANκL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey RANκL. You can calculate the concentration of Monkey RANκL in the samples by comparing the OD of the samples to the standard curve.

Species reactivity:
Monkey

Sample Type: serum, plasma, cell lysates, tissue homogenates, cell culture supernatant or other biological fluids

Storage instruction: -20˚C and 4˚C for 6 months