Monkey VEGFR1/FLT1 (Vascular Endothelial Growth Factor Receptor 1) ELISA Kit

Description:
This ELISA kit applies to the in vitro quantitative determination of Monkey VEGFR1/FLT1 concentrations in serum, plasma and other biological fluids.

Assay Principles: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey VEGFR1/FLT1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey VEGFR1/FLT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey VEGFR1/FLT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzymesubstrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey VEGFR1/FLT1. You can calculate the concentration of Monkey VEGFR1/FLT1 in the samples by comparing the OD of the samples to the standard curve.

Species reactivity:
Monkey

Sample Type: serum, plasma, cell supernatant

Storage instruction: -20˚C and 4˚C for 6 months