Monoclonal Anti-β-Actin loading control antibody

Background:
Beta-actin (gene name ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Karakozova et al. (2006) found that approximately 40% of intracellular beta-actin is arginylated in vivo. In both wildtype and Ate1-null cells beta-actin was stable, suggesting that arginylation does not induce beta-actin degradation. And arginylation of beta-actin apparently represents a critical step in the actin N-terminal processing needed for actin functioning in vivo.1Nitric oxide (NO) is a paracrine mediator of vascular and platelet function that is produced in the vasculature by NO synthase-3. And beta-actin plays a critical role in regulating NO formation and signaling in platelets.2Beta actin is also usually used as a loading control, for among others, the integrity of cells, protein degradation, in PCR and Western Blotting.

Immunogen:
Human β-actin full-length protein

Specificity: Detect the epitope located on N-terminal ofβ-Actin.Western Blot (1:1000-10,000); Other applications have not been tested. The optimal dilutions should be determined by end user.

Product Form: Liquid

Storage Buffer: 0.01 M PBS (PH 7.4), 0.1% BSA, 0.02% sodium azide, 50% glycerol

Storage Instruction:
Store at -20℃ for 1 year. Avoid repeated freeze and thaw cycles.