MRP14/MAC 387/S100A9/Calgranulin B (clone47-8D3), anti-human, rat, bovine, baboon, cat, dog, goat, guinea pig, horse, monkey, pig, rabbit

Source:
A BALB/c mouse was immunized with human peripheral blood monocyte components derived by affinity chromatography with Sepharose 4B coupled to rabbit monocyte antibodies.
Fusion partner: NS-1.

Specifications:
47-8D3 reacts with macrophages and detects the well-known leukocyte L1, cystic fibrosis antigen. Detecting a single protein band of 14 kDa in Western blots of lysates of human monocytes and granulocytes, the antigen was identified as the calcium-binding protein MRP14, which is a member of the S100 family involved a.o. in regulating the cell cycle. MRP14 is also implicated in the abnormal differentiation of myeloid cells in the stroma of cancer. It is further found on squamous mucosal epithelia. When associated with MRP8 it forms the heterodimer calprotectin.

Species reactivity:
Positive: baboon, cat, cow, dog, goat, guinea pig, human, horse, monkey, pig, rabbit, rat.

Applications:
MRP14 is widely used for identifying cystic fibrosis and the phenotypical characterization of macrophages and myelomonocytic cells in situ.
Flow cytometry: +
Frozen section: +s
Immunofluorescence: +
Paraffin sections: Citrate/trypsin
Western blot: +

Positive control: Monocytes, macrophages, granulocytes.

References:
- Flavell DJ. et al., J. Histochem. Cytochem.35: 1217-1226 (1987).
- Facchetti F. et al., Am. J. Clin. Pathol.92: 42-50 (1989).
- Bardadin KA. et al., J. Pathol.164: 253-259 (1991).
- Goebeler M. et al., J. Leukocyte Biol.55: 259-261 (1994)