MSCs-derived exosomes RUO
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In stock
SKU
IS-MSC
PRODUCT DESCRIPTION
Lyophilized/Frozen exosomes (~1x1012 ) from adipose-derived Mesenchymal stem/stromal cells (MSCs) (8,9).
Exosomes are isolated by differential ultracentrifugation (10).
• Tested application: In-vitro functional assay, Flow Cytometry (FMC), Nanoparticles Tracking Analysis (NTA, Nanosight), Western Blot (WB), BCAProtein Assay.
• Species reactivity: Human
• Presentation: Lyophilized/Frozen
Reconstitution of lyophilized Exosomes:
For reconstitution, we recommended adding sterile, distilled water to achieve a final exosome concentration of 1μg/μl (e.g., for 100 μg standard, add 100 μl of dH2O). After the addition of water, recap vial and briefly vortex making sure that the liquid has been gently distributed and has covered the entire inside of the vial.
After vortexing, make sure that the solution is collected at the bottom of the vial, if not, centrifuge shortly the vial solution. Now the standard is ready to use.
PERFORMANCE DATA:
At the time of exosome collection, for each batch, MSCs phenotype analysis is performed by FCM in order, to guarantee the right signature described by The Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy (ISCT) (11). Thus MSC must express CD105, CD73 and CD90, and lack expression of CD45, CD34, CD14 or CD11b, CD79alpha or CD19 and HLA-DR surface molecules.
Lyophilized/Frozen exosomes (~1x1012 ) from adipose-derived Mesenchymal stem/stromal cells (MSCs) (8,9).
Exosomes are isolated by differential ultracentrifugation (10).
• Tested application: In-vitro functional assay, Flow Cytometry (FMC), Nanoparticles Tracking Analysis (NTA, Nanosight), Western Blot (WB), BCAProtein Assay.
• Species reactivity: Human
• Presentation: Lyophilized/Frozen
Reconstitution of lyophilized Exosomes:
For reconstitution, we recommended adding sterile, distilled water to achieve a final exosome concentration of 1μg/μl (e.g., for 100 μg standard, add 100 μl of dH2O). After the addition of water, recap vial and briefly vortex making sure that the liquid has been gently distributed and has covered the entire inside of the vial.
After vortexing, make sure that the solution is collected at the bottom of the vial, if not, centrifuge shortly the vial solution. Now the standard is ready to use.
PERFORMANCE DATA:
At the time of exosome collection, for each batch, MSCs phenotype analysis is performed by FCM in order, to guarantee the right signature described by The Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy (ISCT) (11). Thus MSC must express CD105, CD73 and CD90, and lack expression of CD45, CD34, CD14 or CD11b, CD79alpha or CD19 and HLA-DR surface molecules.
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